HPLC Column, NP Amino, Wide Pore, 10um, 4.0mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.

Additional Information:

Cogent NP Amino Wide Pore is a versatile normal phase and HILIC stationary phase designed for retention of highly polar compounds and larger molecules that can be difficult to retain using conventional reversed-phase columns. Manufactured on high-purity 300 Å wide pore silica with a propyl amino bonded phase, it provides excellent selectivity for complex carbohydrates, sugars, organic acids, and other highly polar analytes. This stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both normal phase and HILIC separations involving larger molecular species.

68040-05P-4-3M is an analytical HPLC column packed with 10 µm NP Amino Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 50 mm format. The short column length is optimized for rapid analyses, fast method development, and screening applications. The 10 µm particle size supports higher flow rates with lower operating backpressure, while the wide pore structure improves accessibility for larger molecules. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, analyte characteristics, and system configuration.

This column is particularly effective for complex sugars, carbohydrates, negatively charged compounds, organic acids, carboxylic acid-containing compounds, and other polar molecules requiring enhanced retention. The wide pore design provides improved performance for larger analytes in both Normal Phase and HILIC applications. As with all unmodified silica-based amino phases, this column is suitable for HILIC applications.

Key Benefits

  • NP Amino Wide Pore stationary phase

  • USP L8 equivalent chemistry

  • Propyl amino bonded phase

  • 300 Å wide pore silica

  • Suitable for Normal Phase chromatography

  • Suitable for HILIC applications

  • Enhanced retention of polar compounds

  • Improved accessibility for larger molecules

  • Low operating backpressure with 10 µm particles

  • Excellent for method development and screening

Typical Applications

  • USP L8 methods

  • HILIC separations

  • Normal Phase chromatography

  • Complex sugar analysis

  • Carbohydrate analysis

  • Organic acid analysis

  • Carboxylic acid analysis

  • Food and beverage testing

  • Pharmaceutical analysis

  • Method development

Specifications

  • Product Type: HPLC Column

  • Stationary Phase: NP Amino Wide Pore

  • USP Classification: L8

  • Chromatography Mode: Normal Phase / HILIC

  • Bonded Phase: Propyl Amino

  • Endcapping: Not Endcapped

  • Particle Size: 10 µm

  • Pore Size: 300 Å

  • Column Dimensions: 4.0 mm ID x 50 mm

  • Length: 50 mm

  • Typical Flow Rate: 0.8–1.5 mL/min

  • Brand: Cogent

Special Order Item Information

Important Notes

  • Packed and shipped in Normal Phase solvents

  • Suitable for both Normal Phase and HILIC chromatographic modes

  • Wide pore design improves access for larger molecules and complex carbohydrates

  • Excellent retention of sugars, carbohydrates, and organic acids

  • Short column length is ideal for rapid analyses and method development

  • This is a Special Order Item


 

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